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PLASTIC RECONSTRUCTIVE AND REGENERATIVE SURGERY

Original article

Ahead-of-Print

Efficiency assessment of cord blood banking and compatibility with delayed cord clamping

Authors

Geethika Sajeewani Manchanayake , Elisenda Farssac Busquets , Ana García Buendia , Patrícia Ferrer Aguilar , Gisela Palomar Centena , Maria José Pelegay Escartín , Irene Ribera Casellas , Carmen Azqueta Molluna , Dinara Samarkanova , Jesus Fernandez-Sojo , Nerea Castillo Flores , Sergio Querol

Key words: Cord blood banking, Delayed Cord Clamping, Donation programme, Cord blood collection
DOI: 10.2450/BloodTransfus.767
Collection, production and storage of blood components
Publication Date: 2024-08-05

Abstract

Background There is debate whether delayed umbilical cord clamping following delivery, the current gold standard, affects the proportion of cord blood units (CBU) suitable for public cord blood banking. This study was designed to assess the impact of delayed cord clamping on the number of CBU suitable for therapeutic uses.

M&M To minimize variability, data from the four most active collection centers within the Programa Concordia (Spain) were included. Data on CBU collected in utero from mothers following normal vaginal deliveries from July 2018 to December 2021 were analyzed. The weight of the collection bags (as a surrogate of volume) and total nucleated cell (TNC) count were analyzed according to three defined clamping times: 30 s, 60 s and ≥120 s. The CBU were stratified as suitable for stem cell transplantation (≥110 g and ≥1,500x106 TNC/unit) or other clinical applications (≥100 g but TNC count below the threshold). 

Results There were 131 (18%), 548 (76%), and 40 (5%) CBU collected at 30 s, 60 s and ≥120 s, respectively. The median weight of the CBU decreased gradually with time, with a significant difference between units collected when the cord was clamped at 30 s or 60 s (p=0.036), so significantly fewer CBU met the minimal weight criterion (100 g) at 60 s than at 30 s (p=0.002). However, this was not reflected by the TNC available, resulting in non-statistical differences in CBU eligible for banking between these times. The major predictor of collection success was the neonate’s birth-weight.

Discussion Despite decreases in the volume of cord blood collected when cord clamping at 30 s or 60 s, TNC count is maintained resulting in similar numbers of CBU eligible for banking. The different clamping delays investigated in this study are, therefore, compatible with public cord blood banking needs.

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Authors

Geethika Sajeewani Manchanayake - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain; Blood Bank, Teaching Hospital, Karapitiya, Sri Lanka https://orcid.org/0000-0002-0247-0714

Elisenda Farssac Busquets - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain; Transfusional Medicine Study Group, Vall de Hebron Research Institute, Barcelona, Spain https://orcid.org/0000-0001-9111-5978

Ana García Buendia - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain

Patrícia Ferrer Aguilar - Obstetrics Department, Sant Joan de Déu d’Esplugues Hospital, Barcelona, Spain

Gisela Palomar Centena - Obstetrics and Gynecology Department. Germans Trias i Pujol Hospital, Barcelona, Spain

Maria José Pelegay Escartín - Obstetrics and Gynecology Department. Arnau de Vilanova Hospital, Barcelona, Spain https://orcid.org/0000-0003-4846-6160

Irene Ribera Casellas - Obstetrics and Gynecology Department. Vic University Hospital, Barcelona, Spain https://orcid.org/0000-0003-4390-7510

Carmen Azqueta Molluna - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain https://orcid.org/0000-0002-9621-0891

Dinara Samarkanova - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain; Transfusional Medicine Study Group, Vall de Hebron Research Institute, Barcelona, Spain

Jesus Fernandez-Sojo - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain; Transfusional Medicine Study Group, Vall de Hebron Research Institute, Barcelona, Spain

Nerea Castillo Flores - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain https://orcid.org/0000-0001-5441-1516

Sergio Querol - Programa Concordia, Banc de Sang i Teixits, Barcelona, Spain https://orcid.org/0000-0002-1105-8633

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