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PLASTIC RECONSTRUCTIVE AND REGENERATIVE SURGERY

Original article

Vol. 22 No. 6 (2024): Blood Transfusion 6-2024 (November-December)

Assessment of metabolic and hemostatic profile of apheresis platelet concentrates: does the storage medium play a role?

Authors

Eleni Petrou , Stavros Tsalas , Andreas G. Tsantes , Electra Loukopoulou , Sofia Mellou , Sotirios P. Fortis , Evdoxia Rapti , Rozeta Sokou , Elias Kyriakou , Panagiota Douramani , Frantzeska Franzteskaki , George Samonis , Styliani Kokoris , Anastasios G. Kriebardis , Argirios E. Tsantes

Key words: Mirasol-treated apheresis platelets, ROTEM, Pathogen Reduction Technology, Storage medium
DOI: 10.2450/BloodTransfus.800
Collection, production and storage of blood components
Publication Date: 2024-07-30

Abstract

Background - The impact of pathogen reduction technology (PRT) on metabolic and haemostatic profile of treated platelets remains a subject of debate. Platelets Additive Solutions (PASs) are suggested as more appropriate storage medium compared to plasma. To investigate this in terms of zero heterogeneity PRT-treated and control apheresis platelet concentrates (PCs), collected from the same donors and stored in PAS and plasma respectively, were analyzed.

Materials and methods - In the first arm of the study six double dose-apheresis PCs were produced, split and stored in plasma, while in the second arm six split double dose-apheresis PCs from the same donors, were produced and stored in PAS. Control and PRT-treated PCs resulted in both arms. Metabolic and haemostatic markers were evaluated in all the examined groups on days 1, 3 and 5.

Results - A time dependent increased metabolism both in PAS and plasma-stored PCs was evident in PRT-treated PCs. However, the metabolic profile was better preserved in PCs stored in PAS, as higher pH (6.8 vs 6.5, p=0.007) and lower lactate levels (12.6 vs 17.8 mmol/L, p=0.009) were documented in PRT-treated PAS-PCs compared to plasma-PCs, on day 5. A time dependent decreased hemostatic capacity regardless the storage medium was evident in PRT-treated PCs, (PAS-PCs MCF, p=0.004 and plasma-PCs MCF, p=0.007). Similar results were obtained in control PCs.

Discussion - The use of PAS preserves the metabolic profile of PCs more adequately compared to plasma but has no effect on the haemostatic profile. The clinical relevance of these findings needs further investigation.

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Authors

Eleni Petrou - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

Stavros Tsalas - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

Andreas G. Tsantes - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece https://orcid.org/0000-0002-3695-0759

Electra Loukopoulou - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

Sofia Mellou - Transfusion Department, General Hospital of Athens "G. Gennimatas", Athens, Greece

Sotirios P. Fortis - Laboratory of Reliability and Quality Control in Laboratory Hematology, Department of Biomedical Science, School of Health and Caring Science, University of West Attica, Athens, Greece

Evdoxia Rapti - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

Rozeta Sokou - Neonatal Intensive Care Unit, "Agios Panteleimon" General Hospital of Nikea, Nikea, Piraeus, Greece

Elias Kyriakou - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

Panagiota Douramani - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

Frantzeska Franzteskaki - 2nd Department of Critical Care Medicine, General University Hospital "Attikon", Medical School, National and Kapodistrian University of Athens, Athens, Greece

George Samonis - Department of Oncology, Metropolitan Hospital, Athens, Greece; Department of Medicine, University of Crete, Heraklion, Greece

Styliani Kokoris - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

Anastasios G. Kriebardis - Laboratory of Reliability and Quality Control in Laboratory Hematology, Department of Biomedical Science, School of Health and Caring Science, University of West Attica, Athens, Greece

Argirios E. Tsantes - Laboratory of Hematology and Blood Bank Unit, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

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